NONYLPHENOL INDUCES EXPRESSION OF THE T-BOX6 GENE IN ZEBRAFISH EMBRYOS: RESULTS(3)

Expression of ntl gene, spt gene and tbx16 gene is shown in Figure 3. Although the zebrafish were harvested within a short period of time the variation within the single groups is remarkably high. Therefore statistical significant differences are seldom observed. Highest expression of ntl gene (38.8±30.6) was seen after exposition the embryos to p252-NP, followed by the expression after treatment with p33-NP (16.8±11.7). The expression of ntl gene in the other zebra fish embryos varied between 7.7±4.4 in p22-NP and 0.8±0.6 in the p353-NP group. Expression of spt gene was highest in zebrafish embryos treated with t-NP embryos (33.3±15.6). The spt gene expression in zebra fish embryos under treatment of the different isomers of nonyphenol used varied between 0.72±0.63 (p353-NP) and 20.9±16.1 (p252-NP). Performing linear regression using the expression of spt gene and nt} gene based on the response of the individual embryo revealed a significant correlation with a coefficient of determination R2= 0.9172.


Fig3Nonylphenol Induces Expression_decrypted
Figure 3 : Gene expression levels of ntl (a), spt (b) and tbx6 (c) relating to the housekeeping genes rpl13a and efla of zebra fish embryos treated with different synthetic isomers of nonylphenols (NP) with a concentration of 10 Nmol/L for 1 hr. Technical NP (t-NP) is a mixture of more than 20 NP isomers. Dimethyl sulfoxide (DMSO) was used as vehicle control. Values are means of 5 independent experiments with SEM. Asterisks indicate significant difference (p<0.05) as compared to DMSO control (Tukey-Kramer test)

The expression of tbx6 gene measured for zebrafish embryos exposed to all isomers but p353-NP and t-NP is comparable to the expression observed in zebrafish embryos treated with DMSO (values between 0.3±0.1 for p252-NP and 2±2 for p33-NP). Zebrafish embryos treated with t-NP showed an expression of 9.8±3.7. A marked increase in tbx6gene expression was observed in zebra fish embryos treated with p353-NP (83.5±14.7). This induction is statistically significant different from the expression of tbx6 gene in zebrafish exposed to DMSO alone.

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